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 Beagle Prototype

 

The principle of the Beagle detector is based upon Raman Spectroscopy induced by a 224 nm laser. The choice of the 224 nm laser was driven by 2 main properties :

- Normal Raman Spectroscopy is typically done in the visible or near infrared, and is quite insensitive compared with fluorescence. This is because normal Raman scatter cross-sections are small (10-30 cm2) compared to fluorescence (10-17cm2).
But when Raman excitation occurs within an electronic resonance (absorption) band of a material, the scatter cross-section can be improved as much as 108. A protein, for example, will typically absorbs at 214 nm, and the 224 nm laser is a good answer to provide a significant resonance enhancement of Raman bands.
 
                                                                                                                   
Beagle

 - There are no fluorescence interference when excitation is provided at the 224 nm wavelengths. A typical Raman spectral range of 4000 cm-1 occurs in less than 30 nm above the excitation wavelength at 224 nm. independent of the excitation wavelength, no known material fluoresces at wavelengths below about 280 nm. This provides complete spectral separation of Raman and fluorescence emission bands resulting in high signal to noise ratio measurements and low detection limits.

                                                                                
beagle screen

The Beagle detector objective is to identify the conformational changes of recombinant proteins synthetized from genes of olfactory cells.

The development was realized within the framework of a contract obtained with the US DOD.

The instrument is a prototype.

It is available with a static detection cell or with an on-column detection cell (capillary).

 
     
beagle preliminary results

 

 
 
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